Analysis at the Sequence Level of Mutations Induced by the Ultimate Carcinogen N-Acetoxy-N-2-Acetylaminofluorene
نویسندگان
چکیده
منابع مشابه
Analysis at the sequence level of mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene
The covalent binding of an ultimate carcinogen to the DNA bases or phosphate groups creates a premutational lesion that in vivo is processed by the repair, replication and recombination enzymes, and eventually may be converted into a mutation. Being interested in the way that an initial premutational event is converted into a stable heritable mutation, we have sequenced stable mutations in a ge...
متن کاملAntibodies to DNA modified by the carcinogen N-acetoxy-N-2-acetylaminofluorene.
Several studies have shown that the carcinogen N-acetoxy-N-2-acetylaminofluorene (AAAF) reacts in vivo and in vitro with native DNA and that the DNA contains a major (80%) adduct N(deoxyguanosin8-yl)-acetylaminofluorene (dGuo8-AAF) and a minor (20%) adduct 3{deoxyguanosin-N2-yl)-acetylaminofluorene (dGuo-N2-AAF) (reviewed [1,2]). It has been shown that the major alteration induced by the fluore...
متن کاملInhibitory effect of reducing agents on N-acetoxy- and N-hydroxy-2-acetylaminofluorene-induced mutagenesis.
The effect of cysteine (alpha-amino-beta-mercaptopropionic acid) on the mutagenic activities of the proximate carcinogen, N-hydroxy-2-acetylaminofluorene, and the ultimate carcinogen, N-acetoxy-2-acetylaminofluorene, was examined by estimating the frequency of his+ revertants of Salmonella typhimurium. Nontoxic concentrations of cysteine significantly reduced the formation of revertants when it...
متن کاملEnzymatic N-hydroxylation of the Carcinogen 2-acetylaminofluorene and the Metabolism of N-hydroxy-2-acetylaminofluorene-9-14c in Vitro.
In 1960, Cramer, Miller, and Miller (1) reported the discovery of a new metabolite of the carcinogen 2-acetylaminofluorene, and from this discovery there has evolved a hypothesis concerning the mechanism of action of carcinogenic aromatic amines (2-5). This metabolite, N-hydrosy-2.acetylaminofluorene, has been found in the urine of animals fed 2-acetylaminofluorene only as a conjugate cleavable...
متن کاملIn Vitro Binding of N-Acetoxy-N-2-acetylaminofluorene in Chromatin to DNA
The binding of N-acetoxy-N-2-acetylaminofluorene to DNA in native and partially dehistonized chicken erythrocyte chromatin was studied. The amounts of carcinogen bound to DNA were measured, after removal of proteins with phenol, by using the absorption ratio A3os/A260 or by counting the radioactivity of 14C-labeled carcinogen. Measurements of uncovered zones of DNA in chromatin were made by com...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1983
ISSN: 0091-6765
DOI: 10.2307/3429590